1. Inicio
  2. Publicaciones
  3. Laboratory and clinical practices in antinuclear antibody detection and related antigens: recommendations from a Spanish multicentre survey

Laboratory and clinical practices in antinuclear antibody detection and related antigens: recommendations from a Spanish multicentre survey

Fecha de publicación: Fecha Ahead of Print:

Autores de IDIVAL

  • Juan Irure Ventura

    Autor

Autores ajenos al IDIVAL

  • Martinez-Martinez L
  • Jurado A
  • Roy G
  • Montes MA
  • Barrios Y
  • Rodríguez-Bayona B
  • Vergara E
  • Prada Á
  • Rojo R
  • Vargas ML
  • GEAI-SEI

Unidades

Abstract

Antinuclear antibodies (ANA) are the most widely used immunological test for the diagnosis of autoimmune diseases. Despite the recommendations of experts, there is some variability in performing and interpreting this test in routine practice. In this context, the Spanish Group on Autoimmune Diseases (GEAI) of the Spanish Society of Immunology (SEI) conducted a national survey of 50 autoimmunity laboratories. Here we report the survey results on ANA testing, detection of related antigens, and our recommendations. The survey showed that most of the participating laboratories use a similar approach for most key practices: 84% perform ANA by indirect immunofluorescence (IIF) on HEp-2 cells as the screening methodology while the other laboratories use IIF to confirm positive screens; 90% report ANA test results as either negative or positive with titer and pattern; 86% indicated that the ANA pattern conditioned follow-up testing for specific antigen-related antibodies; and 70% confirm positive anti-dsDNA. However, testing practices were highly heterogeneous for certain items, such as sera dilutions and the minimum time period for repeating ANA and related antigen determinations. Overall, this survey shows that most autoimmune laboratories in Spain use a similar approach but that further standardization of testing and reporting protocols is needed.

© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

Datos de la publicación

ISSN/ISSNe:
0257-277X, 1559-0755

IMMUNOLOGIC RESEARCH  HUMANA PRESS INC

Tipo:
Article
Páginas:
749-759
PubMed:
37195550

Citas Recibidas en Web of Science: 2

Documentos

  • No hay documentos

Métricas

Filiaciones mostrar / ocultar

Keywords

  • Antinuclear antibodies (ANA); HEp-2 cells; ICAP; Indirect immunofluorescence (IIF); Survey

Compartir