1. Inicio
  2. Publicaciones
  3. Plasma with Added Protease Inhibitors Improves Alpha- and Beta-CGRP Measurement Compared to Serum: Towards a Reliable Biomarker for Chronic Migraine

Plasma with Added Protease Inhibitors Improves Alpha- and Beta-CGRP Measurement Compared to Serum: Towards a Reliable Biomarker for Chronic Migraine

Fecha de publicación:

Autores de IDIVAL

Unidades

Abstract

The neuropeptide calcitonin gene-related peptide (CGRP), especially alpha-CGRP, is central in migraine pathophysiology. Although CGRP is a therapeutic target and potential biomarker, inconsistencies in measurement procedures need to be further studied for reliable results. This study aims to analyze factors influencing plasma CGRP measurement. Chronic migraine (CM) patients were recruited in our Headache Unit. Blood samples were collected before and during treatment with CGRP monoclonal antibodies, processed and stored. Levels of CGRP were measured with isoform-specific enzyme-linked immunosorbent assay (ELISA) tests. Statistical tests were used to assess concentration changes and group differences. The addition of protease inhibitors (PIs) to plasma samples significantly increased alpha-CGRP level detection, with a smaller effect on beta-CGRP. No correlation was found between the alpha- and beta-CGRP levels in plasma. The plasma-PI samples showed higher CGRP concentrations than in serum. The alpha-CGRP levels decreased during treatment while the beta-CGRP levels remained stable. alpha-CGRP and age correlated negatively, but no sex-related differences were observed either for alpha- or beta-CGRP. PI improved CGRP detection in plasma. The alpha-CGRP levels, which were influenced by age, decreased with specific treatment, suggesting its potential role as a biomarker. In contrast, beta-CGRP remained stable, suggesting independent regulation of both isoforms.

Datos de la publicación

ISSN/ISSNe:
1661-6596, 1422-0067

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES  MDPI

Tipo:
Article
Páginas:
-
PubMed:
41155252

Documentos

  • No hay documentos

Métricas

Filiaciones mostrar / ocultar

Keywords

  • CGRP; migraine; protease inhibitors; biomarker; plasma; serum

Campos de Estudio

Proyectos asociados

Especificidad y sensibilidad de los niveles de CGRP: análisis a fondo de su estado en migraña, otras cefaleas primarias y secundarias y en enfermedades inflamatorias sistémicas

Investigador Principal: Julio Pascual Gómez

PI20/01358 . INSTITUTO DE SALUD CARLOS III. . 2021

Medicina de precisión para prevenir la cronificación del la migraña a través de la predicción de crisis y de la respuesta al tratamiento con anticuerpos anti-CGRP utilizando estrategias multi-ómicas

Investigador Principal: Julio Pascual Gómez

PMP22/00183 . 2023

Cita

Compartir